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B.Y. Zaslavsky: Aqueous Two‐Phase Partitioning – Physical Chemistry and Bioanalytical Applications, Marcel Dekker, Inc., New York, Basel, Oxford, ISBN.
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Purifying, Detecting, and Characterizing Proteins. W H Freeman; Partitioning in aqueous two-phase systems: Fundamentals, applications and trends.

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Sep Purif Rev ; A viable platform in the manufacturing of biopharmaceuticals. J Chromatogr A ; High throughput characterization of structural differences between closely related proteins in solution, biochimica et biophysica. Acta Gen Subj ; Partition of Cell Particles and Macromolecules. About a peculiarity of soluble starch. Partition of proteins in a three-phase system.

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Eur J Biochem ; The separation of cells and organelles by partitioning in two-polymer aqueous phases. Purification of enzymes by liquid-liquid extraction. Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses and Applications to Biotechnology. Physical Chemistry and Bioanalytical Applications. Partition of cells in two-polymer aqueous phases: A surface affinity method for cell separation. Walter H, Johansson G. Applications of phase partitioning in biotechnology. Kula MR, Selber K. Protein purification, aqueous liquid extraction.

Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis, and Bioseparation. John Wiley and Sons; Hydrophobic surface properties of erythrocytes studied by affinity partition in aqueous two-phase systems. Mol Cell Biochem ; New aqueous two-phase system based on hydroxypropyl starch useful in enzyme purification.

Enzyme Microb Technol ;8: Vernau J, Kula MR.

Bioanalytical applications of partitioning in aqueous polymer two-phase systems.

Extraction of proteins from biological raw material using aqueous polyethylene glycol-citrate phase systems. Biotechnol Appl Biochem ; It will be not possible to visualize any scientific studies Essay On the internet Simply writing for Newbies September 18, In Uncategorized Read More Academic publishing functions are some of the incredibly typical school assignments because of the usefulness Expert King William is the Antichrist master of just one world government September 18, In Uncategorized Read More Should you not brain, we can assist you to finish youth memory documents by supplying a few parts of guidance It actually is out of the question to imagine any research Phase inversion takes place near the point of , where the continuous phase can change into the dispersed phase, and a sudden change of phase separation behavior is observed.

The bottom phase is continuous when the is less than 0. The top phase is continuous when the is more than 0. It indicates that ATPS would be better to operate in the bottom-continuous region rather than top-continuous region.

Aqueous Two-Phase Systems (ATPS)

The surface tension of water can be used in the equation rather than the surface tension of continuous phase. Hence, 2 can be modified as The value of the coefficients can be calculated with the experimental values Tables 1 and 2. This was performed by nonlinear regression using 1stOpt 1.

Using these values for the bottom-continuous region, 4 can be written as 5 and 6. The correlation index of the two equations is 0. The values of the coefficient indicate the difference of the phase separation rates between the two ATPS. The has a similar effect on the phase separation rates of both ATPS. Both 5 and 6 have predicted the phase separation rates of ATPS with.

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For the batch separation, the results of prediction can provide a reference since the stable ATPS with are widely used. For the continuous separation, such as HSCCC, the phase separation rate is the most important factor for the choice of solvent systems [ 36 ]. Hence, it is necessary to investigate the relationship between phase separation rate and physical properties of ATPS. In this paper, as phase diagrams show in Figure 6 , the bottom-continuous regions of the four investigated ATPS are fit for continuous separation due to the fast phase separation rates and weak dependence.

It can be seen that the maximum value of stationary phase retention was The density of bottom phase is larger than that of top phase and increases with increasing TLL. The viscosity of the top phase is 3—35 times larger than the bottom phase and increases with the increasing TLL.

The interfacial tension between the phases increases with the increasing TLL. The bottom phase is continuous at high value, and fast separation rate has been observed as well as the smooth variation. The top phase is continuous at low value, and the dramatic increase of phase separation time with the increasing value was observed. There is a phase inversion band between the top-continuous region and bottom-continuous region. The phase inversion band is located at the constant salt concentration line in the phase diagram. Within this region, the continuity of the phase is affected not only by the composition of the ATPS, but also by the fluid dynamics.

The phase separation rate was correlated using a modified correlation equation and the coefficients were found. It showed that the of the two equations were 0.

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The correlation equations gave good results for the bottom-continuous ATPS with. The results may be useful for the choice of optimal conditions of ATPS no matter in batch separation or continuous separation process. Height of the interphase: Time of phase separation: Volume of top phase: Volume of bottom phase: